Nan Wang
Xi’an Jiaotong University, China
Title: The MRGPRX2 related pseudo-allergy was inhibited by several compounds from traditional Chinese medicine
Biography
Biography: Nan Wang
Abstract
Pseudo-allergic reactions; adverse, non-immunologic, anaphylaxis-like sudden onset reactions mediated through an IgE-independent pathway are activated by various basic compounds and occur at least as frequently as IgE-mediated reactions to drugs. A large family of G protein coupled receptors (Mas-related genes, MRGPRS) is closely related to pseudo-allergies. However, few therapies can directly target pseudo-allergies and related MRGPRS. Chinese herbal medicine has a long history in China and is still widely used owing to its specific therapeutic effect, broad indications, safety and convenience. Cell Membrane Chromatography (CMC) is an effective new method to directly screen and identify the target components from a complex system and has been widely used in recent years. Isoliquiritigenin (ISO) and Saikosaponin A (SSA) were screen from traditional Chinese medicine injection using a mast cell CMC system. We examined the effect of SSA and ISO on both IgE-independent and IgE-dependent responses using PCA and active systemic anaphylaxis models as well as in vitro cultured mast cells. We also evaluated whether the anti-allergy effect is related to MRGPRS by using in vitro MRGPRX2-expressing HEK293 cells. Both SSA and ISO could also dose-dependently inhibit C48/80-induced MRGPRX2-expressing HEK293 cell activation. This study provided a new sight on pseudo-allergy and its therapy.